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Mycelial lysates were prepared in lysis buffer (100 mM Tris-HCl, 50 mM NaCl, 20 mM EDTA, 10% (v/v) Glycerol, 1 mM PMSF, 1 g/ml pepstatin A, pH 7.5) with grinding, sonication and clarified using centrifugation (13,000 g, 20 min, 4 C)

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Understanding the physiology of natural GLP-1 provides essential context for appreciating why pharmaceutical modifications were necessary to harness its therapeutic potential for chronic disease management, particularly in type 2 diabetes and obesity treatment

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